Hotspot

trnS-GCU-trnG-UCC

LSC · 9300-10322 · 1023 bp

Back to Menispermum

Pi 0.0324

MarkerSeek score 74.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1023
variable_sites: 32
pi: 0.0324
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	AATCAAACCGAAAGACCCCT	50.0	45.0	58.0	57.0	1103-1135 bp	1.0	76.9
trnS-GCU-trnG-UCC_p2	CAATCCGACGCTTTAGTCCA	ATCAAACCGAAAGACCCCTT	50.0	45.0	58.0	57.0	1102-1134 bp	1.0	76.9
trnS-GCU-trnG-UCC_p3	AATCCGACGCTTTAGTCCAC	AATCAAACCGAAAGACCCCT	50.0	45.0	58.0	57.0	1102-1134 bp	1.0	76.9
trnS-GCU-trnG-UCC_p4	AATCCGACGCTTTAGTCCAC	ATCAAACCGAAAGACCCCTT	50.0	45.0	58.0	57.0	1101-1133 bp	1.0	76.9
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	ATCAAACCGAAAGACCCCTT	50.0	45.0	58.2	57.0	1156-1188 bp	1.0	75.5