Hotspot

psbI-trnS-GCU

LSC · 9067-9211 · 145 bp

Back to Menispermum

Pi 0.0345

MarkerSeek score 72.30

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 145
variable_sites: 5
pi: 0.0345
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	AATCCTGGGCGTGAGGAATA	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.5	58.0	239 bp	1.0	77.7
psbI-trnS-GCU_p2	ATCCTGGGCGTGAGGAATAA	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.5	58.0	238 bp	1.0	77.7
psbI-trnS-GCU_p3	TAATCCTGGGCGTGAGGAAT	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.5	58.0	240 bp	1.0	77.7
psbI-trnS-GCU_p4	AATCCTGGGCGTGAGGAATA	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.5	58.0	240 bp	1.0	77.7
psbI-trnS-GCU_p5	ATCCTGGGCGTGAGGAATAA	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.5	58.0	239 bp	1.0	77.7