Hotspot

trnK-UUU-rps16

LSC · 4862-5806 · 945 bp

Back to Menispermum

Pi 0.0145
MarkerSeek score 71.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
945
variable_sites
13
pi
0.0145
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCTACCAACGGTATGGACGA TCCTTGAAAAAGGTGCCCAA 50.0 45.0 57.9 57.8 1112-1157 bp 1.0 81.8
trnK-UUU-rps16_p2 TCTACCAACGGTATGGACGA TTCCTTGAAAAAGGTGCCCA 50.0 45.0 57.9 57.8 1113-1158 bp 1.0 81.8
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TCCTTGAAAAAGGTGCCCAA 50.0 45.0 58.2 57.8 1047-1092 bp 1.0 81.7
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TCCTTGAAAAAGGTGCCCAA 50.0 45.0 58.2 57.8 1046-1091 bp 1.0 81.7
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TTCCTTGAAAAAGGTGCCCA 50.0 45.0 58.2 57.8 1047-1092 bp 1.0 81.7