Hotspot

ndhF-rpl32

SSC · 115658-116498 · 841 bp

Back to Memecylon

Pi 0.0109
MarkerSeek score 66.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
841
variable_sites
9
pi
0.0109
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGTTGCCATTGGAAAAACGA GGAAAAAGCCTTCAACGCTG 40.0 50.0 56.7 58.2 1039-1182 bp 1.0 69.8
ndhF-rpl32_p2 TGTTGCCATTGGAAAAACGA AGGAAAAAGCCTTCAACGCT 40.0 45.0 56.7 58.3 1040-1183 bp 1.0 69.2
ndhF-rpl32_p3 TGTTGCCATTGGAAAAACGA GCCTTCAACGCTGCAAAATA 40.0 45.0 56.7 57.7 1032-1175 bp 1.0 68.9
ndhF-rpl32_p4 TTGTTGCCATTGGAAAAACGA GGAAAAAGCCTTCAACGCTG 38.1 50.0 57.5 58.2 1040-1183 bp 1.0 67.7
ndhF-rpl32_p5 TGTTGCCATTGGAAAAACGA AAGGAAAAAGCCTTCAACGC 40.0 45.0 56.7 57.5 1041-1184 bp 1.0 67.6