Hotspot

ndhK-ndhC

LSC · 52059-52126 · 68 bp

Back to Memecylon

Pi 0.0476

MarkerSeek score 70.20

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 68
variable_sites: 3
pi: 0.0476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	GGGGATTTTTATGGGATTCA	GCGAAAAGGAGCATTGGAAT	40.0	45.0	52.8	57.1	112-117 bp	1.0	45.6
ndhK-ndhC_p2	GGGGATTTTTATGGGATTCA	GGCGAAAAGGAGCATTGGAA	40.0	50.0	52.8	59.1	113-118 bp	1.0	45.5
ndhK-ndhC_p3	GGGGATTTTTATGGGATTCA	CATGGCGAAAAGGAGCATTG	40.0	50.0	52.8	58.1	116-121 bp	1.0	45.4
ndhK-ndhC_p4	GGGGATTTTTATGGGATTCAT	CATGGCGAAAAGGAGCATTG	38.1	50.0	53.5	58.1	116-121 bp	1.0	45.4
ndhK-ndhC_p5	GGGGATTTTTATGGGATTCA	TATGCATGGCGAAAAGGAGC	40.0	50.0	52.8	59.0	120-125 bp	1.0	45.2