Hotspot

trnC-GCA-ndhJ

LSC · 49885-50739 · 855 bp

Back to Memecylon

Pi 0.00587

MarkerSeek score 62.50

Primer available yes

Label trnC-GCA-ndhJ

Feature Metadata

label_name: trnC-GCA-ndhJ
feature_type: igs
region: LSC
length_bp: 855
variable_sites: 5
pi: 0.00587
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ACTCATCCACGACTGAAACG	55.0	50.0	58.0	57.9	1030-1033 bp	1.0	80.1
trnC-GCA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	CTCATCCACGACTGAAACGT	55.0	50.0	58.0	57.9	1029-1032 bp	1.0	80.1
trnC-GCA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ACTCATCCACGACTGAAACG	50.0	50.0	58.1	57.9	994-997 bp	1.0	79.7
trnC-GCA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	CTCATCCACGACTGAAACGT	50.0	50.0	58.1	57.9	993-996 bp	1.0	79.7
trnC-GCA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	TGAAAGTTGGCTAGGATGGC	55.0	50.0	58.0	57.9	998-1001 bp	1.0	79.6