Hotspot

trnT-GGU-psbD

LSC · 32551-33931 · 1381 bp

Back to Memecylon

Pi 0.00437

MarkerSeek score 61.80

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1381
variable_sites: 6
pi: 0.00437
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.1	58.0	1516-1523 bp	1.0	80.2
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1519-1526 bp	1.0	80.0
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.3	58.0	1493-1500 bp	1.0	78.5
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1524-1531 bp	1.0	78.3
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	GACCGGACCAACCTACAAAA	55.0	50.0	58.1	57.7	1538-1545 bp	1.0	78.1