Hotspot

trnS-GCU-trnR-UCU

LSC · 8058-9865 · 1808 bp

Back to Memecylon

Pi 0.00566
MarkerSeek score 63.50
Primer available yes
Label trnS-GCU-trnR-UCU

Feature Metadata

label_name
trnS-GCU-trnR-UCU
feature_type
igs
region
LSC
length_bp
1808
variable_sites
10
pi
0.00566
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnR-UCU_p1 CAATCCGACGCTTTAGTCCA AGAACTTGCGTCCAATAGGA 50.0 45.0 58.0 56.6 1882-1923 bp 1.0 70.6
trnS-GCU-trnR-UCU_p2 AATCCGACGCTTTAGTCCAC AGAACTTGCGTCCAATAGGA 50.0 45.0 58.0 56.6 1881-1922 bp 1.0 70.6
trnS-GCU-trnR-UCU_p3 CAATCCGACGCTTTAGTCCA AAGAACTTGCGTCCAATAGGA 50.0 42.9 58.0 57.3 1883-1924 bp 1.0 68.5
trnS-GCU-trnR-UCU_p4 AATCCGACGCTTTAGTCCAC AAGAACTTGCGTCCAATAGGA 50.0 42.9 58.0 57.3 1882-1923 bp 1.0 68.5
trnS-GCU-trnR-UCU_p5 AGCAATCCGACGCTTTAGTC AGAACTTGCGTCCAATAGGA 50.0 45.0 58.4 56.6 1884-1925 bp 1.0 67.7