Hotspot

syfB

Genome · 40160-42163 · 2004 bp

Back to Membranoptera

Pi 0.0145

MarkerSeek score 52.00

Primer available yes

Label syfB

Feature Metadata

label_name: syfB
feature_type: gene
region: Genome
length_bp: 2004
variable_sites: 29
pi: 0.0145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
syfB_p1	GGGTGTAGCCAAGTGGTAAG	AAGTTTGAGGAAAGTCCGGG	55.0	50.0	57.9	57.7	2956 bp	1.0	78.0
syfB_p2	GGTGTAGCCAAGTGGTAAGG	AAGTTTGAGGAAAGTCCGGG	55.0	50.0	57.9	57.7	2955 bp	1.0	78.0
syfB_p3	GAATCCTCCCACCCCAGATA	AAGTTTGAGGAAAGTCCGGG	55.0	50.0	57.9	57.7	2903 bp	1.0	77.9
syfB_p4	GGGTGTAGCCAAGTGGTAAG	AAGTCCGGGCTCTCTATAGG	55.0	55.0	57.9	57.7	2945 bp	1.0	77.8
syfB_p5	GGTGTAGCCAAGTGGTAAGG	AAGTCCGGGCTCTCTATAGG	55.0	55.0	57.9	57.7	2944 bp	1.0	77.8