Hotspot

orf121-apcF

Genome · 149112-150131 · 1020 bp

Back to Membranoptera

Pi 0.0257

MarkerSeek score 61.20

Primer available yes

Label orf121-apcF

Feature Metadata

label_name: orf121-apcF
feature_type: igs
region: Genome
length_bp: 1020
variable_sites: 26
pi: 0.0257
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf121-apcF_p1	TGGCACTCTATCCACTGAGT	GCAGCACTCATAATTCCCCA	50.0	50.0	57.8	57.9	2114-2118 bp	1.0	79.5
orf121-apcF_p2	TGGCACTCTATCCACTGAGT	TGCAGCACTCATAATTCCCC	50.0	50.0	57.8	57.9	2115-2119 bp	1.0	79.5
orf121-apcF_p3	TGGCACTCTATCCACTGAGT	ATTGTAGGTGCAAGTGGGAC	50.0	50.0	57.8	57.8	1808-1813 bp	1.0	78.6
orf121-apcF_p4	TGGCACTCTATCCACTGAGT	GTAGGTGCAAGTGGGACATT	50.0	50.0	57.8	57.8	1805-1810 bp	1.0	78.5
orf121-apcF_p5	TGGCACTCTATCCACTGAGT	CCCAATCTCCTGTTTGTGCA	50.0	50.0	57.8	58.4	2098-2102 bp	1.0	76.9