Hotspot

rpl6-rps8

Genome · 73655-73687 · 33 bp

Back to Membranoptera

Pi 0.0606
MarkerSeek score 70.00
Primer available yes
Label rpl6-rps8

Feature Metadata

label_name
rpl6-rps8
feature_type
igs
region
Genome
length_bp
33
variable_sites
2
pi
0.0606
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl6-rps8_p1 GTTTGCTTTAACATTATTCGGT AGGTGGAGAAATACTGTGTT 31.8 40.0 53.7 53.9 118 bp 1.0 41.4
rpl6-rps8_p2 TGTTTGCTTTAACATTATTCGGT AGGTGGAGAAATACTGTGTT 30.4 40.0 55.4 53.9 119 bp 1.0 41.3
rpl6-rps8_p3 ATGTTTGCTTTAACATTATTCGGT AGGTGGAGAAATACTGTGTT 29.2 40.0 55.9 53.9 120 bp 1.0 41.3
rpl6-rps8_p4 TGTTTGCTTTAACATTATTCGG AGGTGGAGAAATACTGTGTT 31.8 40.0 53.7 53.9 119 bp 1.0 41.3
rpl6-rps8_p5 TGTTTGCTTTAACATTATTCGGT TAGGTGGAGAAATACTGTGT 30.4 40.0 55.4 53.0 120 bp 1.0 41.3