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tnrF-GAA-clpC

Genome · 52308-52377 · 70 bp

Back to Membranoptera

Pi 0.0714

MarkerSeek score 69.60

Primer available yes

Label tnrF-GAA-clpC

Feature Metadata

label_name: tnrF-GAA-clpC
feature_type: igs
region: Genome
length_bp: 70
variable_sites: 5
pi: 0.0714
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tnrF-GAA-clpC_p1	TCAGTTGGTAGAGCAGTGGA	CGCCATACAGTCTCAAAGCT	50.0	50.0	58.0	58.0	843 bp	0.5	59.8
tnrF-GAA-clpC_p2	TGGTAGAGCAGTGGACTGAA	CGCCATACAGTCTCAAAGCT	50.0	50.0	58.0	58.0	838 bp	0.5	59.8
tnrF-GAA-clpC_p3	TTGGTAGAGCAGTGGACTGA	CGCCATACAGTCTCAAAGCT	50.0	50.0	58.0	58.0	839 bp	0.5	59.8
tnrF-GAA-clpC_p4	AGTTGGTAGAGCAGTGGACT	CGCCATACAGTCTCAAAGCT	50.0	50.0	58.0	58.0	841 bp	0.5	59.7
tnrF-GAA-clpC_p5	ATTAGCCGGGATAGCTCAGT	CGCCATACAGTCTCAAAGCT	50.0	50.0	58.0	58.0	858 bp	0.5	59.7