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trnH-GUG-ycf29

Genome · 43813-43869 · 57 bp

Back to Membranoptera

Pi 0.0702

MarkerSeek score 72.70

Primer available yes

Label trnH-GUG-ycf29

Feature Metadata

label_name: trnH-GUG-ycf29
feature_type: igs
region: Genome
length_bp: 57
variable_sites: 4
pi: 0.0702
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-ycf29_p1	TTGTGACTCCATCATACGCG	AGAAATCGTACAGAGTTAGTTCA	50.0	34.8	58.1	55.1	138-139 bp	1.0	42.3
trnH-GUG-ycf29_p2	TTGTGACTCCATCATACGCG	TCAAGAAATCGTACAGAGTTAGT	50.0	34.8	58.1	55.1	141-142 bp	1.0	42.3
trnH-GUG-ycf29_p3	TTGTGACTCCATCATACGCG	CAAGAAATCGTACAGAGTTAGTTCA	50.0	36.0	58.1	57.1	140-141 bp	1.0	42.3
trnH-GUG-ycf29_p4	ATCATACGCGGGTTCGATTC	ACAAAATCAAGAAATCGTACAGAGT	50.0	32.0	58.2	57.3	137-138 bp	1.0	42.3
trnH-GUG-ycf29_p5	TTGTGACTCCATCATACGCG	ACAAAATCAAGAAATCGTACAGAGT	50.0	32.0	58.1	57.3	147-148 bp	1.0	42.2