Hotspot

rps1-ycf40

Genome · 43439-43460 · 22 bp

Back to Membranoptera

Pi 0.0909

MarkerSeek score 71.50

Primer available yes

Label rps1-ycf40

Feature Metadata

label_name: rps1-ycf40
feature_type: igs
region: Genome
length_bp: 22
variable_sites: 2
pi: 0.0909
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps1-ycf40_p1	AGGAGATAGTACAGCAGGCT	CGCGTATGATGGAGTCACAA	50.0	50.0	57.2	58.1	1016 bp	1.0	74.5
rps1-ycf40_p2	AGGAGATAGTACAGCAGGCT	GAATCGAACCCGCGTATGAT	50.0	50.0	57.2	58.2	1026 bp	1.0	73.4
rps1-ycf40_p3	AGGAGATAGTACAGCAGGCT	ATTTAAGGGCGAATGACGGG	50.0	50.0	57.2	58.3	1045 bp	1.0	72.5
rps1-ycf40_p4	AGGAGATAGTACAGCAGGCT	GAGTCACAACCCATTGCCTT	50.0	50.0	57.2	58.4	1005 bp	1.0	72.1
rps1-ycf40_p5	AGGAGATAGTACAGCAGGCT	ATGGAGTCACAACCCATTGC	50.0	50.0	57.2	58.5	1008 bp	1.0	71.5