Hotspot

ycf46-rrfB

Genome · 25332-25344 · 13 bp

Back to Membranoptera

Pi 0.0769

MarkerSeek score 75.00

Primer available yes

Label ycf46-rrfB

Feature Metadata

label_name: ycf46-rrfB
feature_type: igs
region: Genome
length_bp: 13
variable_sites: 1
pi: 0.0769
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf46-rrfB_p1	ACAAAAATGGATAACCTCAGGT	CCACTCCAAACCATTCCGAA	36.4	50.0	55.6	58.1	148 bp	1.0	44.5
ycf46-rrfB_p2	ACAAAAATGGATAACCTCAGGT	ATTAATAGCGCAGTGGACCC	36.4	50.0	55.6	57.7	166 bp	1.0	43.2
ycf46-rrfB_p3	ACAAAAATGGATAACCTCAGGT	ACCATTCCGAACTTGGTTGT	36.4	45.0	55.6	57.6	139 bp	1.0	42.4
ycf46-rrfB_p4	ACAAAAATGGATAACCTCAGGT	GACCCACTCCAAACCATTCC	36.4	55.0	55.6	58.5	151 bp	1.0	41.6
ycf46-rrfB_p5	ACAAAAATGGATAACCTCAGGT	GGACCCACTCCAAACCATTC	36.4	55.0	55.6	58.5	152 bp	1.0	41.6