Hotspot

psaF-orf174

Genome · 21542-22386 · 845 bp

Back to Membranoptera

Pi 0.0142

MarkerSeek score 52.10

Primer available yes

Label psaF-orf174

Feature Metadata

label_name: psaF-orf174
feature_type: igs
region: Genome
length_bp: 845
variable_sites: 12
pi: 0.0142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaF-orf174_p1	AACCTCTACCAACCCATCCA	GCTACCAAGCTGCGCTATAT	50.0	50.0	57.9	57.9	1539-1549 bp	1.0	80.2
psaF-orf174_p2	ACCTCTACCAACCCATCCAA	GCTACCAAGCTGCGCTATAT	50.0	50.0	57.9	57.9	1538-1548 bp	1.0	80.2
psaF-orf174_p3	CCATCCAATCCATCCAGCAA	GCTACCAAGCTGCGCTATAT	50.0	50.0	57.9	57.9	1526-1536 bp	1.0	80.1
psaF-orf174_p4	AACCTCTACCAACCCATCCA	GGCCTTCTTACCCATCCAAC	50.0	55.0	57.9	58.2	1115-1125 bp	1.0	79.0
psaF-orf174_p5	ACCTCTACCAACCCATCCAA	GGCCTTCTTACCCATCCAAC	50.0	55.0	57.9	58.2	1114-1124 bp	1.0	79.0