Hotspot

ycf1

SSC · 124803-130217 · 5415 bp

Back to Melodinus

Pi 0.00148

MarkerSeek score 42.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5415
variable_sites: 8
pi: 0.00148
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGATTTTGATGCAGGACTCA	TGGATCGAGCTTTCAAGTACA	42.9	42.9	57.7	57.0	222 bp	1.0	54.4
ycf1_p2	TCGATTTTGATGCAGGACTCA	TGGATCGAGCTTTCAAGTACAA	42.9	40.9	57.7	57.7	222 bp	1.0	51.9
ycf1_p3	TCGATTTTGATGCAGGACTCA	ATGGATCGAGCTTTCAAGTACA	42.9	40.9	57.7	57.5	223 bp	1.0	50.3
ycf1_p4	CGATTTTGATGCAGGACTCA	TGGATCGAGCTTTCAAGTACA	45.0	42.9	56.1	57.0	221 bp	1.0	50.2
ycf1_p5	CGATTTTGATGCAGGACTCAA	TGGATCGAGCTTTCAAGTACA	42.9	42.9	56.9	57.0	221 bp	1.0	48.2