Hotspot

trnN-GUU-trnR-ACG

IRa · 130618-131187 · 570 bp

Back to Melodinus

Pi 0.00702

MarkerSeek score 62.20

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 570
variable_sites: 4
pi: 0.00702
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	711 bp	1.0	78.7
trnN-GUU-trnR-ACG_p2	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	662 bp	1.0	77.5
trnN-GUU-trnR-ACG_p3	CGATTGGTCGTAGGTTCGAA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.7	57.8	666 bp	1.0	76.6
trnN-GUU-trnR-ACG_p4	TCTCCCGTTGTTCCTCAGTA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.7	57.8	715 bp	1.0	76.3
trnN-GUU-trnR-ACG_p5	TTCCTCAGTAGCTCAGTGGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.7	57.8	705 bp	1.0	76.2