Hotspot

ycf1-trnN-GUU

IRa · 130218-130545 · 328 bp

Back to Melodinus

Pi 0.00305

MarkerSeek score 46.70

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 328
variable_sites: 1
pi: 0.00305
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	ATAGAGTCCGACCACAACGA	AGGATTCGAACCTACGACCA	50.0	50.0	58.2	58.2	474 bp	1.0	77.6
ycf1-trnN-GUU_p2	ACCACAACGACCGAATTGAT	AGGATTCGAACCTACGACCA	45.0	50.0	57.8	58.2	464 bp	1.0	77.5
ycf1-trnN-GUU_p3	AGAGGGCCCTATGGAGAATG	AGGATTCGAACCTACGACCA	55.0	50.0	58.3	58.2	510 bp	1.0	76.8
ycf1-trnN-GUU_p4	GCCCTATGGAGAATGTGGTC	AGGATTCGAACCTACGACCA	55.0	50.0	57.7	58.2	505 bp	1.0	76.8
ycf1-trnN-GUU_p5	ATAGAGTCCGACCACAACGA	CCCCAAGTAGGATTCGAACC	50.0	55.0	58.2	57.7	482 bp	1.0	76.2