Hotspot

accD-psaI

LSC · 59995-60619 · 625 bp

Back to Melodinus

Pi 0

MarkerSeek score 59.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 625
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCTCCACGCTTTCTTTCCTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.8	58.0	759-765 bp	1.0	74.3
accD-psaI_p2	GCTCCACGCTTTCTTTCCTT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.8	57.9	765-771 bp	1.0	73.7
accD-psaI_p3	GCTCCACGCTTTCTTTCCT	CCATTGCAATTGCCGGAAAT	52.6	45.0	58.1	58.0	759-765 bp	1.0	71.9
accD-psaI_p4	TCCACGCTTTCTTTCCTTTGA	CCATTGCAATTGCCGGAAAT	42.9	45.0	58.1	58.0	757-763 bp	1.0	71.8
accD-psaI_p5	GCTCCACGCTTTCTTTCCT	AAGAAGCCATTGCAATTGCC	52.6	45.0	58.1	57.9	765-771 bp	1.0	71.3