Hotspot

ndhC-trnV-UAC

LSC · 51429-52697 · 1269 bp

Back to Melodinus

Pi 0.00236

MarkerSeek score 60.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1269
variable_sites: 3
pi: 0.00236
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1400 bp	1.0	77.5
ndhC-trnV-UAC_p2	CTGGTCCTTTGCTAATCGGG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.3	57.8	1417 bp	1.0	75.9
ndhC-trnV-UAC_p3	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	1412 bp	1.0	75.9
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1399 bp	1.0	74.7
ndhC-trnV-UAC_p5	TCCTTTGCTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.5	57.8	1413 bp	1.0	74.6