Hotspot

trnC-GCA-petN

LSC · 28107-28981 · 875 bp

Back to Melodinus

Pi 0.00114

MarkerSeek score 59.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 875
variable_sites: 1
pi: 0.00114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	985 bp	1.0	79.4
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TTCTTCCCCACACTACGAGT	50.0	50.0	58.1	58.0	983 bp	1.0	79.4
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	986 bp	1.0	78.8
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	931 bp	1.0	78.2
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	TTCTTCCCCACACTACGAGT	50.0	50.0	58.4	58.0	1001 bp	1.0	77.2