Hotspot

trnK-UUU-rps16

LSC · 4289-5029 · 741 bp

Back to Melodinus

Pi 0.00135
MarkerSeek score 59.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
741
variable_sites
1
pi
0.00135
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC GGTGCTCAACCTACAGGAAC 55.0 55.0 57.9 58.2 926-928 bp 1.0 77.9
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 865-867 bp 1.0 77.2
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 864-866 bp 1.0 77.2
trnK-UUU-rps16_p4 TCAGTCGTGGTCTTCCAAAC GGTGCTCAACCTACAGGAAC 50.0 55.0 57.8 58.2 950-952 bp 1.0 76.7
trnK-UUU-rps16_p5 CAGTCGTGGTCTTCCAAACT GGTGCTCAACCTACAGGAAC 50.0 55.0 57.8 58.2 949-951 bp 1.0 76.6