Hotspot

petB

LSC · 73617-75092 · 1476 bp

Back to Melocalamus

Pi 0.000904

MarkerSeek score 59.40

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1476
variable_sites: 2
pi: 0.000904
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GGTAGTTCGACCGTGGAATT	ACCACAACCTACCCGATATGA	50.0	47.6	57.6	58.2	1583-1585 bp	1.0	67.2
petB_p2	GGTAGTTCGACCGTGGAATT	ACCACAACCTACCCGATATG	50.0	50.0	57.6	56.7	1583-1585 bp	1.0	66.8
petB_p3	GGTAGTTCGACCGTGGAATT	TGAAATACCACAACCTACCCG	50.0	47.6	57.6	57.7	1589-1591 bp	1.0	66.4
petB_p4	ATTCTGGTAGTTCGACCGTG	ACCACAACCTACCCGATATGA	50.0	47.6	57.4	58.2	1588-1590 bp	1.0	65.0
petB_p5	CATTCTGGTAGTTCGACCGT	ACCACAACCTACCCGATATGA	50.0	47.6	57.4	58.2	1589-1591 bp	1.0	65.0