Hotspot

ndhF

SSC · 104925-107276 · 2352 bp

Back to Melocalamus

Pi 0.00142

MarkerSeek score 57.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2352
variable_sites: 5
pi: 0.00142
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAGGGGAATAAGGGGCTCTT	ACCAAGTAAACCCATTCTTCA	50.0	38.1	58.0	55.0	2455-2456 bp	1.0	48.3
ndhF_p2	AGGGGAATAAGGGGCTCTTT	ACCAAGTAAACCCATTCTTCA	50.0	38.1	58.0	55.0	2454-2455 bp	1.0	48.3
ndhF_p3	TAGGAAGGGGAATAAGGGGC	ACCAAGTAAACCCATTCTTCA	55.0	38.1	58.2	55.0	2459-2460 bp	1.0	46.9
ndhF_p4	AAGGGGAATAAGGGGCTCTT	AACCAAGTAAACCCATTCTTCA	50.0	36.4	58.0	55.8	2456-2457 bp	1.0	46.6
ndhF_p5	AGGGGAATAAGGGGCTCTTT	AACCAAGTAAACCCATTCTTCA	50.0	36.4	58.0	55.8	2455-2456 bp	1.0	46.6