Hotspot

ndhF-rpl32

SSC · 107277-108042 · 766 bp

Back to Melocalamus

Pi 0.000871

MarkerSeek score 51.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 766
variable_sites: 1
pi: 0.000871
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTTCCGATTCACCAAACCA	TCCAAAGATTTTTACGAATACGCT	42.9	33.3	58.3	57.3	910-911 bp	1.0	40.2
ndhF-rpl32_p2	TGTTTCCGATTCACCAAACCA	TGACATCGAAGTACGTTTTT	42.9	35.0	58.3	53.2	882-883 bp	1.0	40.2
ndhF-rpl32_p3	TGTTTCCGATTCACCAAACCA	TTGACATCGAAGTACGTTTT	42.9	35.0	58.3	53.2	883-884 bp	1.0	40.2
ndhF-rpl32_p4	TGTTTCCGATTCACCAAACCA	TTTGACATCGAAGTACGTTT	42.9	35.0	58.3	53.2	884-885 bp	1.0	40.2
ndhF-rpl32_p5	TGTTTCCGATTCACCAAACCA	TTTTGACATCGAAGTACGTT	42.9	35.0	58.3	53.2	885-886 bp	1.0	40.2