Hotspot

atpI-atpH

LSC · 33379-34198 · 820 bp

Back to Melocalamus

Pi 0.00325

MarkerSeek score 48.30

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 820
variable_sites: 4
pi: 0.00325
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TAGCCGCAGCCTATATAGGT	GCAGTACCTTGACCAACTCC	50.0	55.0	57.5	58.2	956 bp	1.0	74.6
atpI-atpH_p2	TAGCCGCAGCCTATATAGGT	AAGCAGCAGCAATTAGTGGA	50.0	45.0	57.5	57.8	889 bp	1.0	74.5
atpI-atpH_p3	TAGCCGCAGCCTATATAGGT	GCCAATCCAGCAGCAATAAC	50.0	50.0	57.5	57.8	911 bp	1.0	74.4
atpI-atpH_p4	TAGCCGCAGCCTATATAGGT	ATAACGGAAGCAGCAGCAAT	50.0	45.0	57.5	58.2	896 bp	1.0	74.2
atpI-atpH_p5	ATTTTTGCAACGTTAGCCGC	GCAGTACCTTGACCAACTCC	45.0	55.0	58.6	58.2	969 bp	1.0	74.1