Hotspot

petD

LSC · 75545-76735 · 1191 bp

Back to Melissa

Pi 0.0118

MarkerSeek score 66.70

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1191
variable_sites: 21
pi: 0.0118
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	CCCTTCAACCAATCGTGTCA	TCCCTAGATACACACGTCGT	50.0	50.0	58.1	57.6	1304 bp	1.0	77.5
petD_p2	TCCCTTCAACCAATCGTGTC	TCCCTAGATACACACGTCGT	50.0	50.0	57.8	57.6	1305 bp	1.0	76.9
petD_p3	TTCCCTTCAACCAATCGTGT	TCCCTAGATACACACGTCGT	45.0	50.0	57.4	57.6	1306 bp	1.0	73.1
petD_p4	CCCTTCAACCAATCGTGTCA	GCTTTGAAGCGACTATTCCC	50.0	50.0	58.1	56.9	1320 bp	1.0	71.8
petD_p5	TCCCTTCAACCAATCGTGTC	GCTTTGAAGCGACTATTCCC	50.0	50.0	57.8	56.9	1321 bp	1.0	71.2