Hotspot

ccsA-ndhD

SSC · 113338-113571 · 234 bp

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Pi 0.0199
MarkerSeek score 56.10
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
234
variable_sites
7
pi
0.0199
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 ACAAATTCAGCAATTGTAGCGT TCAGTTGAAAAGGTTGAAGT 36.4 35.0 57.5 52.8 414 bp 1.0 41.4
ccsA-ndhD_p2 ACAAATTCAGCAATTGTAGCGT TCGTTCTTTCACTATCAGTTGA 36.4 36.4 57.5 55.2 428 bp 1.0 41.3
ccsA-ndhD_p3 ACAAATTCAGCAATTGTAGCGT TCGTTCTTTCACTATCAGTTGAA 36.4 34.8 57.5 55.9 428 bp 1.0 41.3
ccsA-ndhD_p4 ACAAATTCAGCAATTGTAGCGT TTCGTTCTTTCACTATCAGTTGA 36.4 34.8 57.5 55.9 429 bp 1.0 41.3
ccsA-ndhD_p5 ACAAATTCAGCAATTGTAGCGT TCACTATCAGTTGAAAAGGTTGA 36.4 34.8 57.5 55.8 420 bp 1.0 41.3