Hotspot

rpl32-trnL-UAG

SSC · 111445-112183 · 739 bp

Back to Melissa

Pi 0.00722
MarkerSeek score 57.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
739
variable_sites
8
pi
0.00722
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TCTTTCTACCGGGACTTCCA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.7 57.9 833-840 bp 1.0 77.8
rpl32-trnL-UAG_p2 TCTTTCTACCGGGACTTCCA AGCAGCGTGTCTACCAATTT 50.0 45.0 57.7 57.8 826-833 bp 1.0 77.4
rpl32-trnL-UAG_p3 TCTTTCTACCGGGACTTCCA CCTAAGAGCAGCGTGTCTAC 50.0 55.0 57.7 57.8 832-839 bp 1.0 77.3
rpl32-trnL-UAG_p4 TCTTTCTACCGGGACTTCCA CTAAGAGCAGCGTGTCTACC 50.0 55.0 57.7 57.8 831-838 bp 1.0 77.3
rpl32-trnL-UAG_p5 ACCGGGACTTCCAAAAGTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.6 57.9 826-833 bp 1.0 76.8