Hotspot

atpH-atpI

LSC · 13438-14451 · 1014 bp

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Pi 0.00197

MarkerSeek score 60.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1014
variable_sites: 3
pi: 0.00197
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.1	58.3	1147-1148 bp	1.0	77.4
atpH-atpI_p2	AGCCAATCCAGCAGCAATAA	TCCCTGTCATGTTCCTTGGA	45.0	50.0	57.9	58.3	1165-1166 bp	1.0	77.0
atpH-atpI_p3	GCCAATCCAGCAGCAATAAC	TCCCTGTCATGTTCCTTGGA	50.0	50.0	57.8	58.3	1164-1165 bp	1.0	76.5
atpH-atpI_p4	TCCAGCAGCAATAACGGAAG	TCCCTGTCATGTTCCTTGGA	50.0	50.0	58.3	58.3	1159-1160 bp	1.0	76.1
atpH-atpI_p5	AACGGAAGCAGCAGAAATCA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.1	58.5	1099-1100 bp	1.0	75.3