Hotspot

ycf1

SSC · 129913-135597 · 5685 bp

Back to Meliosma

Pi 0.195

MarkerSeek score 75.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5685
variable_sites: 1072
pi: 0.195
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGGGAATGCACATTTCGTTG	TGTCTTTGTGCACGACAGAA	50.0	45.0	58.0	58.0	910 bp	0.5	59.8
ycf1_p2	TCGCCCCGATCCTTATTACT	TGTCTTTGTGCACGACAGAA	50.0	45.0	58.0	58.0	784 bp	0.5	59.8
ycf1_p3	GGGGAATGCACATTTCGTTG	TGCCGAAGTGATGGAAAACA	50.0	45.0	58.0	58.0	992 bp	0.5	59.8
ycf1_p4	TCGCCCCGATCCTTATTACT	TGCCGAAGTGATGGAAAACA	50.0	45.0	58.0	58.0	866 bp	0.5	59.8
ycf1_p5	GGGGAATGCACATTTCGTTG	GGATTTCTTGCTCGGGATGT	50.0	50.0	58.0	58.0	785 bp	0.5	59.6