Hotspot

rpl16

LSC · 86393-87851 · 1459 bp

Back to Meliosma

Pi 0.166

MarkerSeek score 83.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1459
variable_sites: 228
pi: 0.166
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ATTGGGCACTGCTTCTTTGA	AGGTCGAGTTCCCCTACAAA	45.0	50.0	58.0	58.0	2001 bp	0.5	59.8
rpl16_p2	TGGGCACTGCTTCTTTGATT	AAGGTCGAGTTCCCCTACAA	45.0	50.0	58.0	58.0	2000 bp	0.5	59.8
rpl16_p3	TGGGCACTGCTTCTTTGATT	AGGTCGAGTTCCCCTACAAA	45.0	50.0	58.0	58.0	1999 bp	0.5	59.8
rpl16_p4	TTGGGCACTGCTTCTTTGAT	AAGGTCGAGTTCCCCTACAA	45.0	50.0	58.0	58.0	2001 bp	0.5	59.8
rpl16_p5	TTGGGCACTGCTTCTTTGAT	AGGTCGAGTTCCCCTACAAA	45.0	50.0	58.0	58.0	2000 bp	0.5	59.8