Hotspot

psbE-petL

LSC · 69660-71062 · 1403 bp

Back to Meliosma

Pi 0.193

MarkerSeek score 83.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1403
variable_sites: 243
pi: 0.193
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AAAGGATCAAAACGGCCAGT	GGTTCAAGTCCTACAGAGCG	45.0	55.0	58.0	58.0	2145 bp	0.5	59.9
psbE-petL_p2	AAGGATCAAAACGGCCAGTT	GGTTCAAGTCCTACAGAGCG	45.0	55.0	58.0	58.0	2144 bp	0.5	59.9
psbE-petL_p3	AAAGGATCAAAACGGCCAGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	2198 bp	0.5	59.8
psbE-petL_p4	AAGGATCAAAACGGCCAGTT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	2197 bp	0.5	59.8
psbE-petL_p5	AAGGATCAAAACGGCCAGTT	TATAGTCGAAGCCGCCAGTA	45.0	50.0	58.0	58.0	1671 bp	0.5	59.7