Hotspot

petA-psbJ

LSC · 67795-68886 · 1092 bp

Back to Meliosma

Pi 0.249

MarkerSeek score 82.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1092
variable_sites: 245
pi: 0.249
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TAATGGCAAAAAGGGGGCTT	AACTGGCCGTTTTGATCCTT	45.0	45.0	58.0	58.0	2341 bp	0.5	59.7
petA-psbJ_p2	TAATGGCAAAAAGGGGGCTT	ACTGGCCGTTTTGATCCTTT	45.0	45.0	58.0	58.0	2340 bp	0.5	59.7
petA-psbJ_p3	GGGGGCTTTGAATGTAGGAG	AACTGGCCGTTTTGATCCTT	55.0	45.0	57.9	58.0	2329 bp	0.5	59.5
petA-psbJ_p4	GGGGGCTTTGAATGTAGGAG	ACTGGCCGTTTTGATCCTTT	55.0	45.0	57.9	58.0	2328 bp	0.5	59.5
petA-psbJ_p5	GTTTCAGAGGGCGAATCGAT	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.1	58.0	1883 bp	0.5	59.5