Pi
0.278
MarkerSeek score
80.10
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GTAATTGCCGCTCTGTCAGA | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.0 | 58.0 | 1634 bp | 0.5 | 59.9 |
| trnT-UGU-trnL-UAA_p2 | TAATTGCCGCTCTGTCAGAC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.0 | 58.0 | 1633 bp | 0.5 | 59.9 |
| trnT-UGU-trnL-UAA_p3 | AGTAATTGCCGCTCTGTCAG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.0 | 58.0 | 1635 bp | 0.5 | 59.8 |
| trnT-UGU-trnL-UAA_p4 | GTAATTGCCGCTCTGTCAGA | ATACAGACTCGGGTCGTCAT | 50.0 | 50.0 | 58.0 | 57.9 | 1976 bp | 0.5 | 59.5 |
| trnT-UGU-trnL-UAA_p5 | TAATTGCCGCTCTGTCAGAC | ATACAGACTCGGGTCGTCAT | 50.0 | 50.0 | 58.0 | 57.9 | 1975 bp | 0.5 | 59.5 |