Hotspot

ycf3-trnS-GGA

LSC · 48383-49262 · 880 bp

Back to Meliosma

Pi 0.2

MarkerSeek score 83.70

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 880
variable_sites: 166
pi: 0.2
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	ATGAACTGACAGGGGCTAGT	CCCGAGATCTTATTACGGCG	50.0	55.0	58.0	58.0	2228 bp	0.5	59.5
ycf3-trnS-GGA_p2	ATGAACTGACAGGGGCTAGT	GGAGCCCGGCAATTAGTTAA	50.0	50.0	58.0	58.0	2307 bp	0.5	59.3
ycf3-trnS-GGA_p3	TCAACGGTCTTCGATGGTTC	CCCGAGATCTTATTACGGCG	50.0	55.0	57.9	58.0	2071 bp	0.5	59.2
ycf3-trnS-GGA_p4	ATGAACTGACAGGGGCTAGT	TGTGGTCAGTAACGTTGGTG	50.0	50.0	58.0	58.1	1741 bp	0.5	59.2
ycf3-trnS-GGA_p5	CAACGGTCTTCGATGGTTCT	CCCGAGATCTTATTACGGCG	50.0	55.0	57.9	58.0	2070 bp	0.5	59.2