Hotspot

trnE-UUC-trnT-GGU

LSC · 33848-34675 · 828 bp

Back to Meliosma

Pi 0.271

MarkerSeek score 83.20

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 828
variable_sites: 201
pi: 0.271
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GTGCTCTGACCGATTGAACT	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	1562-1585 bp	1.0	91.3
trnE-UUC-trnT-GGU_p2	AGGCAGTTCTGAAGCACAAA	GAACCGATGACTTACGCCTT	45.0	50.0	57.9	58.0	1156 bp	0.5	59.5
trnE-UUC-trnT-GGU_p3	AGGCAGTTCTGAAGCACAAA	GACAAATTCTTGCGCAGTCC	45.0	50.0	57.9	58.0	1615 bp	0.5	59.3
trnE-UUC-trnT-GGU_p4	CTCATGATGGATCGGCGAAT	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	1122 bp	0.5	59.2
trnE-UUC-trnT-GGU_p5	GTGCTCTGACCGATTGAACT	GACAAATTCTTGCGCAGTCC	50.0	50.0	57.9	58.0	2021 bp	0.5	59.1