Hotspot

trnS-GCU-trnG-UCC

LSC · 9346-10444 · 1099 bp

Back to Meliosma

Pi 0.303

MarkerSeek score 81.30

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1099
variable_sites: 272
pi: 0.303
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	TCCACTCAGCCATCTCTCC	ACGAATCACACTTTTACCACT	57.9	38.1	58.1	55.5	1118-1152 bp	1.0	55.0
trnS-GCU-trnG-UCC_p2	TCCACTCAGCCATCTCTCC	GAACGAATCACACTTTTACCACT	57.9	39.1	58.1	57.3	1120-1154 bp	1.0	53.9
trnS-GCU-trnG-UCC_p3	TCCACTCAGCCATCTCTCC	AACGAATCACACTTTTACCACT	57.9	36.4	58.1	56.2	1119-1153 bp	1.0	53.1
trnS-GCU-trnG-UCC_p4	TCCACTCAGCCATCTCTCC	TACCACTAAACTATACCCGC	57.9	45.0	58.1	54.0	1104-1138 bp	1.0	52.0
trnS-GCU-trnG-UCC_p5	TCCACTCAGCCATCTCTCC	GAACGAATCACACTTTTACCAC	57.9	40.9	58.1	56.0	1120-1154 bp	1.0	52.0