Hotspot

rps18

LSC · 67116-67607 · 492 bp

Back to Melinis

Pi 0.00203

MarkerSeek score 43.90

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 492
variable_sites: 1
pi: 0.00203
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	TCGACGGGGATTTCGAGATA	GACGACGAACAAAAATGCGT	50.0	45.0	58.0	58.0	1448-1452 bp	1.0	80.5
rps18_p2	AGAATCGCCACAATACACCC	GACGACGAACAAAAATGCGT	50.0	45.0	58.0	58.0	1417-1421 bp	1.0	80.4
rps18_p3	TCGACGGGGATTTCGAGATA	TCGTCGTTTGTGGATCACTC	50.0	50.0	58.0	57.9	1312-1316 bp	1.0	80.3
rps18_p4	AAAGGAGGTTCATGGCCAAG	GACGACGAACAAAAATGCGT	50.0	45.0	58.1	58.0	1537-1541 bp	1.0	80.2
rps18_p5	GAAAGGAGGTTCATGGCCAA	GACGACGAACAAAAATGCGT	50.0	45.0	58.1	58.0	1538-1542 bp	1.0	80.1