Hotspot

clpP

LSC · 69151-69801 · 651 bp

Back to Melinis

Pi 0.00461

MarkerSeek score 61.40

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 651
variable_sites: 3
pi: 0.00461
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCGATCTTAACCTGATGATTCATCA	ACTGTGGATTCTTTCTCTTCCA	36.0	40.9	57.9	57.0	843 bp	0.5	20.0
clpP_p2	TCGATCTTAACCTGATGATTCA	ACTGTGGATTCTTTCTCTTCCA	36.4	40.9	54.7	57.0	843 bp	0.5	20.0
clpP_p3	AGATCGATCTTAACCTGATGATTCA	ACTGTGGATTCTTTCTCTTCCA	36.0	40.9	57.7	57.0	846 bp	0.5	20.0
clpP_p4	ATTTACAAGAAATCCAGCCA	ACTGTGGATTCTTTCTCTTCCA	35.0	40.9	52.7	57.0	754 bp	0.5	20.0
clpP_p5	CGATCTTAACCTGATGATTCATCA	ACTGTGGATTCTTTCTCTTCCA	37.5	40.9	56.7	57.0	842 bp	0.5	20.0