Hotspot

ccsA

SSC · 108348-109322 · 975 bp

Back to Melinis

Pi 0.00923

MarkerSeek score 62.10

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 975
variable_sites: 9
pi: 0.00923
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GCGGCATTCTCGAAAAAGAA	AGATGTTCAAGGGGTTCTCA	45.0	45.0	57.4	56.0	1134 bp	1.0	60.1
ccsA_p2	GCGGCATTCTCGAAAAAGAA	AAGATGTTCAAGGGGTTCTCA	45.0	42.9	57.4	56.8	1135 bp	1.0	58.4
ccsA_p3	GCGGCATTCTCGAAAAAGAA	AGATGTTCAAGGGGTTCTCAA	45.0	42.9	57.4	56.8	1134 bp	1.0	58.4
ccsA_p4	GCGGCATTCTCGAAAAAGAA	TGTTCAAGGGGTTCTCAAATCA	45.0	40.9	57.4	57.8	1131 bp	1.0	58.3
ccsA_p5	GCGGCATTCTCGAAAAAGAA	AGATGTTCAAGGGGTTCTCAAA	45.0	40.9	57.4	57.5	1134 bp	1.0	56.1