Hotspot

ndhI-ndhA

SSC · 113652-113744 · 93 bp

Back to Melinis

Pi 0.0215

MarkerSeek score 64.90

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 93
variable_sites: 2
pi: 0.0215
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	TGACCAATGTATCTTGCGGC	ACCCAGGATGAGAATGGATCA	50.0	47.6	58.6	58.2	273 bp	1.0	65.9
ndhI-ndhA_p2	CGCGTATTGTTTGTTGACCA	ACCCAGGATGAGAATGGATCA	45.0	47.6	57.3	58.2	254 bp	1.0	65.5
ndhI-ndhA_p3	GCGCGTATTGTTTGTTGACC	ACCCAGGATGAGAATGGATCA	50.0	47.6	58.7	58.2	255 bp	1.0	65.5
ndhI-ndhA_p4	GGCGCGTATTGTTTGTTGAC	ACCCAGGATGAGAATGGATCA	50.0	47.6	58.7	58.2	256 bp	1.0	65.5
ndhI-ndhA_p5	TGACCAATGTATCTTGCGGC	CCCAGGATGAGAATGGATCAG	50.0	52.4	58.6	57.6	272 bp	1.0	64.3