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atpI-atpH

LSC · 33273-34065 · 793 bp

Back to Melinis

Pi 0.00761

MarkerSeek score 63.70

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 793
variable_sites: 6
pi: 0.00761
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	CAACCTTAGCTGCAGCCTAT	CAGTACCTTGACCAACTCCG	50.0	55.0	57.7	57.9	934-935 bp	1.0	77.6
atpI-atpH_p2	CAACCTTAGCTGCAGCCTAT	GCAGTACCTTGACCAACTCC	50.0	55.0	57.7	58.2	935-936 bp	1.0	76.7
atpI-atpH_p3	CAACCTTAGCTGCAGCCTAT	AAGCAGCAGCAATTAGTGGA	50.0	45.0	57.7	57.8	868-869 bp	1.0	76.7
atpI-atpH_p4	CAACCTTAGCTGCAGCCTAT	GCCAATCCAGCAGCAATAAC	50.0	50.0	57.7	57.8	890-891 bp	1.0	76.6
atpI-atpH_p5	CAACCTTAGCTGCAGCCTAT	ATAACGGAAGCAGCAGCAAT	50.0	45.0	57.7	58.2	875-876 bp	1.0	76.3