Hotspot

trnY-GUA-trnD-GUC

LSC · 16681-17039 · 359 bp

Back to Melinis

Pi 0.0139

MarkerSeek score 56.40

Primer available yes

Label trnY-GUA-trnD-GUC

Feature Metadata

label_name: trnY-GUA-trnD-GUC
feature_type: igs
region: LSC
length_bp: 359
variable_sites: 5
pi: 0.0139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnD-GUC_p1	AATTCTTCCTGGGTCGATGC	CGGTGCTCTGACCAATTGAA	50.0	50.0	58.0	58.5	483 bp	1.0	76.7
trnY-GUA-trnD-GUC_p2	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	55.0	50.0	58.4	58.5	450 bp	1.0	74.3
trnY-GUA-trnD-GUC_p3	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.6	58.5	449 bp	1.0	74.1
trnY-GUA-trnD-GUC_p4	AATTCTTCCTGGGTCGATGC	GGTGCTCTGACCAATTGAAC	50.0	50.0	58.0	57.0	482 bp	1.0	72.5
trnY-GUA-trnD-GUC_p5	TGGGGACGGACTGTAAATTC	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.2	58.5	451 bp	1.0	70.9