Hotspot

trnK-UUU-rps16

LSC · 3965-4546 · 582 bp

Back to Melinis

Pi 0.00935

MarkerSeek score 54.40

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 582
variable_sites: 5
pi: 0.00935
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCACTTAAAAGCCGAG	ATAAAGAACGAACTTTGAGTTAA	50.0	26.1	58.6	52.4	612-658 bp	1.0	44.6
trnK-UUU-rps16_p2	TAGTCGCACTTAAAAGCCGA	ATAAAGAACGAACTTTGAGTTAA	45.0	26.1	57.3	52.4	613-659 bp	1.0	44.6
trnK-UUU-rps16_p3	TCGCACTTAAAAGCCGAGTA	ATAAAGAACGAACTTTGAGTTAA	45.0	26.1	57.3	52.4	610-656 bp	1.0	44.6
trnK-UUU-rps16_p4	CGCACTTAAAAGCCGAGTAC	ATAAAGAACGAACTTTGAGTTAA	50.0	26.1	57.2	52.4	609-655 bp	1.0	44.6
trnK-UUU-rps16_p5	GTCGCACTTAAAAGCCGAGT	ATAAAGAACGAACTTTGAGTTAA	50.0	26.1	58.9	52.4	611-657 bp	1.0	44.6