Hotspot

trnL-CAA

Genome · 86605-86685 · 81 bp

Back to Melilotus

Pi 0.643

MarkerSeek score 74.90

Primer available yes

Label trnL-CAA

Feature Metadata

label_name: trnL-CAA
feature_type: tRNA
region: Genome
length_bp: 81
variable_sites: 45
pi: 0.643
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA_p1	CTCGGGATGACATGACGATC	GTGATCTGCTGGCGAACTTA	55.0	50.0	57.9	58.0	243 bp	0.5	59.5
trnL-CAA_p2	CTCGGGATGACATGACGATC	TCTGCTGGCGAACTTATTCC	55.0	50.0	57.9	58.0	239 bp	0.5	59.3
trnL-CAA_p3	CTCGGGATGACATGACGATC	CTGCTGGCGAACTTATTCCT	55.0	50.0	57.9	58.0	238 bp	0.5	59.3
trnL-CAA_p4	CTCGGGATGACATGACGATC	ACGGGGTGAGATTTGTCCTA	55.0	50.0	57.9	58.1	545 bp	0.5	59.1
trnL-CAA_p5	CTCGGGATGACATGACGATC	CTTTCTCGTCGTCTCATGGG	55.0	55.0	57.9	58.1	666 bp	0.5	58.9