Hotspot

rps18

Genome · 63285-63662 · 378 bp

Back to Melilotus

Pi 0.0596

MarkerSeek score 80.50

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: Genome
length_bp: 378
variable_sites: 22
pi: 0.0596
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	TCTTACAACGCAACAACCTCA	AGAAATGGGATTTTCGGTATAGGA	42.9	37.5	58.1	57.5	528-530 bp	1.0	44.7
rps18_p2	TCTTACAACGCAACAACCTCA	TGGGATTTTCGGTATAGGAA	42.9	40.0	58.1	53.4	523-525 bp	1.0	44.7
rps18_p3	CTTACAACGCAACAACCTCA	AGAAATGGGATTTTCGGTATAGGA	45.0	37.5	56.6	57.5	527-529 bp	1.0	44.7
rps18_p4	TCTTACAACGCAACAACCTCA	ATGGGATTTTCGGTATAGGA	42.9	40.0	58.1	53.1	524-526 bp	1.0	44.7
rps18_p5	TCTTACAACGCAACAACCTCA	GAAATGGGATTTTCGGTATAGGA	42.9	39.1	58.1	56.1	527-529 bp	1.0	44.7