Hotspot

rrn4.5-rrn5

Genome · 105792-106021 · 230 bp

Back to Melilotus

Pi 0.087

MarkerSeek score 76.00

Primer available yes

Label rrn4.5-rrn5

Feature Metadata

label_name: rrn4.5-rrn5
feature_type: igs
region: Genome
length_bp: 230
variable_sites: 20
pi: 0.087
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn4.5-rrn5_p1	CAGCTGAGGCATCCTAACAG	TATCGTCACCGCAGTAGAGT	55.0	50.0	58.0	58.0	345 bp	1.0	84.0
rrn4.5-rrn5_p2	GCTGAGGCATCCTAACAGAC	TATCGTCACCGCAGTAGAGT	55.0	50.0	58.1	58.0	343 bp	1.0	84.0
rrn4.5-rrn5_p3	CAGCTGAGGCATCCTAACAG	AGTATCGTCACCGCAGTAGA	55.0	50.0	58.0	58.0	347 bp	1.0	83.9
rrn4.5-rrn5_p4	GCTGAGGCATCCTAACAGAC	AGTATCGTCACCGCAGTAGA	55.0	50.0	58.1	58.0	345 bp	1.0	83.9
rrn4.5-rrn5_p5	CAGCTGAGGCATCCTAACAG	TCCCCTACAGTATCGTCACC	55.0	55.0	58.0	57.9	355 bp	1.0	83.6