Hotspot

ycf2-trnI-CAU

Genome · 92814-92929 · 116 bp

Back to Melilotus

Pi 0.544

MarkerSeek score 74.20

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: Genome
length_bp: 116
variable_sites: 62
pi: 0.544
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	AAACCCTGTAAATGGCGTGA	GAACCTACGACTTCGCCAAT	45.0	50.0	57.7	58.0	409 bp	0.5	57.7
ycf2-trnI-CAU_p2	AACCCTGTAAATGGCGTGAA	ATTGAACCTACGACTTCGCC	45.0	50.0	57.7	58.0	411 bp	0.5	57.7
ycf2-trnI-CAU_p3	AACCCTGTAAATGGCGTGAA	GAACCTACGACTTCGCCAAT	45.0	50.0	57.7	58.0	408 bp	0.5	57.7
ycf2-trnI-CAU_p4	ACCCTGTAAATGGCGTGAAA	ATTGAACCTACGACTTCGCC	45.0	50.0	57.7	58.0	410 bp	0.5	57.7
ycf2-trnI-CAU_p5	ACCCTGTAAATGGCGTGAAA	GAACCTACGACTTCGCCAAT	45.0	50.0	57.7	58.0	407 bp	0.5	57.7